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Useful image analysis software to measure cell and tissue morphological changes. Many software packages allow researchers to quantifiy kinematic and morphometric data from live images. This table describes advantages and disadvantages of specific software used for general quantitative image analysis and software with specific uses for cell segmentation which allows data for individual cells to be derived in semi-automated or automated fashion and typically used with confocal images of apical cell surfaces. To our knowledge no single method is completely error-free and must therefore allow the user to manually correct erroneously segmented cells.

Journal: Birth defects research

Article Title: Mechanics of neurulation: from classical to current perspectives on the physical mechanics that shape, fold, and form the neural tube

doi: 10.1002/bdra.23557

Figure Lengend Snippet: Useful image analysis software to measure cell and tissue morphological changes. Many software packages allow researchers to quantifiy kinematic and morphometric data from live images. This table describes advantages and disadvantages of specific software used for general quantitative image analysis and software with specific uses for cell segmentation which allows data for individual cells to be derived in semi-automated or automated fashion and typically used with confocal images of apical cell surfaces. To our knowledge no single method is completely error-free and must therefore allow the user to manually correct erroneously segmented cells.

Article Snippet: SIESTA ( Fernandez-Gonzalez and Zallen, 2011 ) , Watershed Algorithm Cell Segmentation Software , Semi-automated segmentation- allows some user correction Built-in GUI Outputs data on select morphological parameters Accounts for surface curvature Matlab, DIPImage ToolBox based Provides 2D analysis Not open-source.

Techniques: Software, Derivative Assay, Cell Tracking Assay